Journal:

Article Title: Protein Kinase C- α and ERK1/2 Mediate Mitochondrial Dysfunction, Decreases in Active Na + Transport, and Cisplatin-induced Apoptosis in Renal Cells

doi: 10.1074/jbc.M206373200

Figure Lengend Snippet: The monolayers were treated and RPTC functions analyzed as described under “Experimental Procedures.” White columns, controls; black columns, 50 μm cisplatin; light gray columns, 50 μm PD98059 + 50 μm cisplatin; dark gray columns, 10 nm Go6976 + 50 μm cisplatin; hatched columns, 3 μm UO126 + 50 μm cisplatin; striped columns, 50 μm zVAD-fmk + 50 μm cisplatin. Results are the average ± S.E. of three to five independent experiments (RPTC isolations).

Article Snippet: PKC- α inhibitor (Go6976) and MEK inhibitors (PD98059 and UO126) were supplied by Calbiochem (La Jolla, CA). zVAD-fmk was purchased from Biomol (Plymouth Meeting, PA).

Techniques:

Journal: Nature Communications

Article Title: Cancer cells dying from ferroptosis impede dendritic cell-mediated anti-tumor immunity

doi: 10.1038/s41467-022-31218-2

Figure Lengend Snippet: a Prophylactic vaccination model assessing the immunogenicity of ferroptosis. ML162-induced ferroptosis (5 μM, 14 h), in comparison with apoptosis (1000 IU/ml TNF + 10 μM TAK1i, 14 h) and necroptosis (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h). OVA-expressing non-tumorigenic MEF cells (BM1-OVA) were used as a vaccine and live ovalbumin expressing melanoma cells (B16-OVA) were used as challenge. Kaplan–Meier curves represent the effectiveness of ferroptotic cells in preventing the tumor growth at the challenge site. Data were analyzed by Kaplan–Meier simple survival analysis. b Tumor size of B16-OVA derived melanoma after vaccination with BM1-OVA cells. Data presented as mean ± SEM. c Scheme of the therapeutic vaccination experiment. Conventional dendritic cells type 1 (cDC1) carrying ferroptotic or necroptotic cargo were intradermally injected in melanoma tumor-bearing mice. d B16-OVA tumor size progression of animals receiving either cDC1 with ferroptotic (5 μM, 14 h) or necroptotic (1000 IU/ml TNF + 10 μM TAK1i + 10 μM zVAD.fmk, 24 h) cargo. Data presented as mean ± SEM. Statistical significance was determined by two-sided t-test on each day of measurement. e Comparison of euthanasia time determined by the size of the tumor. Kaplan–Meier curves show the time of euthanasia, Data analyzed by Kaplan–Meier simple survival analysis. f Prophylactic vaccination model using either Mitoxantrone-treated MCA205 cells (1 μM, 24 h) or Mitoxantrone-treated MCA205 mixed with ML162-killed cells (0.5 µM, 14 h). Kaplan–Meier curves show the percentage of tumor-free mice after the challenge with live cancer cells. Data were analyzed by Kaplan–Meier simple survival analysis.

Article Snippet: Cell death inhibitors zVAD.fmk (#BACE4026865.0005, VWR International, Belgium) and Nec-1s (synthesized by the Laboratory of Medicinal Chemistry; University of Antwerp, Belgium) were used at 10 μM, Fer1 (#M60042-2s, Xcess Biosciences, USA) was used at 0,5 μM and DFO (#D-9533, Sigma-Aldrich, USA) at 50 μM.

Techniques: Expressing, Derivative Assay, Injection